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Original Article
- Optimized Protocols for the Isolation and Culture of Primary Neurons from Rat Cortex, Hippocampus, Spinal Cord, and Dorsal Root Ganglia
- Ganchimeg Davaa, Do In Kim, Jennifer O. Buitrago, Jin Young Hong
- Perspect Integr Med. 2025;4(1):16-27. Published online February 21, 2025
- DOI: https://doi.org/10.56986/pim.2025.02.003
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- 2 Citations
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Abstract
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Supplementary Material - Background
The isolation and culture of primary neurons from specific regions of the rat nervous system are fundamental techniques for investigating neuronal function, development, and pathology. These tools allow the exploration of distinct neural populations and their roles in health and disease.
Methods
Protocols were optimized for dissection, isolation, and culture of primary neurons from the rat cortex, hippocampus, spinal cord, and dorsal root ganglia. Each methodology was customized to address the unique properties of the respective tissue types, focusing on key steps to enhance neuronal yield and viability whilst minimizing contamination with non-neuronal cells. The protocols incorporate refined enzymatic dissociation techniques, mechanical trituration methods, and specialized culture conditions to support neuronal survival and maturation. Additionally, essential considerations for neuronal culture such as growth medium composition, cell density used for plating, and substrate preparation were addressed.
Results
These region-specific methodologies yielded robust and reproducible outcomes, enabling the generation of reliable in vitro models of neurons from both the central and peripheral nervous systems. The optimized procedures effectively increased neuronal viability and purity, making them suitable for a wide range of neuroscience applications.
Conclusion
This comprehensive set of protocols represents a valuable resource for researchers working in neuroscience on rats. Practical approaches to isolate and culture neurons from diverse regions of the nervous system in the rat have been described. The methodologies outlined provide a strong foundation for studying neuronal populations and their significance in various physiological and pathological contexts. -
Citations
Citations to this article as recorded by
- Neurons in a Dish: A Review of In Vitro Cell Models for Studying Neurogenesis
Mariana Vassal, Ana C. Cruz, Sandra Rebelo, Filipa Martins
Journal of Neurochemistry.2026;[Epub] CrossRef - Cibotii Rhizoma extract protects rat dorsal root ganglion neurons against H2O2-induced oxidative stress
Hyunseong Kim, Jin Young Hong, Changhwan Yeo, Hyun Kim, Wan-Jin Jeon, Junseon Lee, Yoon Jae Lee, Eugene Huh, In-Hyuk Ha
BMC Complementary Medicine and Therapies.2025;[Epub] CrossRef
- Neurons in a Dish: A Review of In Vitro Cell Models for Studying Neurogenesis
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